Molecular Authentication of the Traditional Medicinal Plant Fallopia multiflora

Abstract

The root of Fallopia multiflora is one of the most widely used traditional Chinese medicines. However, it is often confused and substituted with the roots of F. multiflora var. ciliinervis, Pteroxygonum giraldii, Cynanchum auriculatum, and Stephania cepharantha. To establish a DNA polymorphism-based assay for the identification of F. multiflora, the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) regions of six Fallopia species were sequenced and analyzed. Based on the diversity of ITS regions among the species the diagnostic primers PMITS28 and PMITS545, which amplified an expected 517-bp DNA fragment from F. multiflora DNA, were designed. No amplified product was observed when DNA from other species was used. This method can be used for the authentication of F. multiflora.

Fallopia multiflora - Polygonaceae - nrDNA ITS region - allele-specific PCR - molecular authentication

References

• 1 Chiu P Y, Mak D H, Poon M K, Ko K M. In vivo antioxidant action of a lignan-enriched extract of Schisandra fruit and an anthraquinone-containing extract of Polygonum root in comparison with schisandrin B and emodin.  Planta Med. 2002;  68 951-956
• 2 Ryu G, Ju J H, Park Y J, Ryu S Y, Choi B W, Lee B H. The radical scavenging effects of stilbene glucosides from Polygonum multiflorum.  Arch Pharm Res. 2002;  25 636-639
• 3 Kimura Y, Okusa H. Effects of naturally occurring stilbene glucosides from medicinal plants and wine, on tumour growth and lung metastasis in Lewis lung carcinoma-bearing mice.  J Pharm Pharmacol. 2000;  52 1287-1295
• 4 Um M Y, Choi W H, Aan J Y, Kim S R, Ha T Y. Protective effect of Polygonum multiflorum Thunb on amyloid β-peptide 25–35 induced cognitive deficits in mice.  J Ethnopharmacol. 2006;  104 144-148
• 5 Chan Y C, Cheng F C, Wang M F. Beneficial effects of different Polygonum multiflorum Thunb. extracts on memory and hippocampus morphology.  J Nutr Sci Vitaminol (Tokyo). 2002;  48 491-497
• 6 Li R W, David Lin G, Myers S P, Leach D N. Anti-inflammatory activity of Chinese medicinal vine plants.  J Ethnopharmacol. 2003;  85 61-67
• 7 Zhao L, Li Y L, Zhang L, Jin H T, Li L. Effects of tetrahydroxystilbene-glucoside on rat model of β-amyloid increase induced by hypercholesterol.  Chin Pharmacol Bull. 2005;  21 49-52
• 8 Zhang M, Xu D R, Zhang Z J, Wang Z T. Identification and quality analysis on commercial herbs of Radix Polygoni Multiflori.  Chin Pharm J. 2005;  40 823-826
• 9 Joshi K, Chavan P, Warude D, Patwardhan B. Molecular markers in herbal drug technology.  Curr Sci. 2004;  87 159-165
• 10 Carles M, Lee T, Moganti S, Lenigk R, Tsim K W, Ip N Y, Hsing I M, Sucher N J. Chips and Qi: microcomponent-based analysis in traditional Chinese medicine.  Fresenius J Anal Chem. 2001;  371 190-194
• 11 Xue C Y, Li D Z, Lu J M, Yang J B, Liu J Q. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii. .  Planta Med. 2006;  72 721-726
• 12 Li X, Ding X, Chu B, Ding G, Gu S, Qian L, Wang Y, Zhou Q. Molecular authentication of Alisma orientale by PCR-RFLP and ARMS.  Planta Med. 2007;  73 67-70
• 13 Ma L, Qiang S. Identification and classification of cut-flower “Huangyinghua” using DNA marker techniques.  Acta Phytotaxon Sin. 2007;  45 497-504