Abstract
The root of Fallopia multiflora is one of the most widely used traditional Chinese medicines. However, it is often
confused and substituted with the roots of F. multiflora var. ciliinervis, Pteroxygonum giraldii, Cynanchum auriculatum, and Stephania cepharantha. To establish a DNA polymorphism-based assay for the identification of F. multiflora, the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) regions of six
Fallopia species were sequenced and analyzed. Based on the diversity of ITS regions among
the species the diagnostic primers PMITS28 and PMITS545, which amplified an expected
517-bp DNA fragment from F. multiflora DNA, were designed. No amplified product was observed when DNA from other species
was used. This method can be used for the authentication of F. multiflora.
Key words
Fallopia multiflora
- Polygonaceae - nrDNA ITS region - allele-specific PCR - molecular authentication
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Prof. Dr. Shu-Jin Zhao
Department of Pharmacy
Liuhuaqiao Hospital
Liuhua Road 111
Guangzhou 510010
Guangdong Province
People's Republic of China
Phone: + 86 20 36 65 34 77
Fax: + 86 20 36 65 34 77
Email: gzzsjzhs@163.com